A modified orcinol reaction for RNA determination.

A Modified Orcinol Reaction for RNA Determination

Studies on the Estimation of Deoxyribonucleic Acid and Ribonucleic Acid in Rat Brain

A method for the estimation of DNA and RNA in rat brain is described. The principal modifications of preexisting methods involve initial lipid extraction with chloroformmethanol and continuous stirring during alkaline hydrolysis of RNA and acid extraction of DNA. Comparison of ultraviolet-absorptive methods, phosphate determination, and orcinol reaction for RNA, shows a greater degree of corres...

The fluorometric determination of malic acid.

A sensitive and specific chemical method for the estimation of malic acid in biological materials has long been desired. The condensation of malic acid with orcinol in the presence of concentrated sulfuric acid to form the highly fluorescent homoumbelliferone (7-hydroxyd-methylcoumarin) was reported years ago (1). Although this reaction is relatively specific for malic acid, the quantitative pr...

Determination of nucleic acids in animal tissues.

As reported in a previous paper (l), a new microchemical method for the determination of deoxyribonucleic acid (DNA) has been devised which permits the evaluation of DNA in amounts as small as 2.5 y per ml. The method is particularly useful with very small quantities of tissue. For this reason a study was undertaken to learn the best conditions for the application of the method to the determina...

A sensitive method for the determination of ribonucleic acid in tissues and microorganisms.

The increasing interest in the composition of nucleoproteins has emphasized the need for more specific methods of assay of constituent nucleic acids. In a previous paper (1) a method was reported for the assay of deoxyribonucleic acid (DNA) in tissues and microorganisms based on the reaction of p-nitrophenylhydrazine with deoxyribose following hydrolysis of the biological material with 5 per ce...